Fig. 1

Monitoring of the stability of molecular alterations in GB cell cultures. a-c Representative figures showing results of conducted molecular analyses: FISH result presenting chromosome 7 trisomy and EGFR amplification in single cells of GB7 in passage 1 of monolayer culture (a); MLPA analysis showing CDKN2A deletion in GB5 in passage 3 of monolayer conditions (b); IDH1 sequencing of GB4 – the line marks the mutated nucleotide in codon 132 (R132H) (c); Changes in CDKN2A status (d), EGFR^vIII and EGFR^WT DNA copy number (e) and EGFR^WT and EGFR^vIII mRNA expression (f) in the culture course of glioblastoma cells in two analyzed culture conditions. EGFR probe (red signals); CEP 7 control probe – centromere of chromosome 7, enabling to show the number of chromosomes 7 (green signals). For MLPA results error bars indicate SD, while for Real-time PCR SEM. Statistical significance for Real-time PCR results was determined by two-way ANOVA analysis with post-analysis Bonferroni’s multiple comparisons test to compare each sample to the adequate frozen sample. Results were considered statistically significant at * p < 0.05; ** p < 0.01; *** p < 0.005