Fig. 7
Expression of CD73 and emmprin in epithelioid sarcoma. The hematoxylin and eosin (H&E) section shows proliferation of severely atypical polygonal cells with enlarged hyperchromatic nuclei, forming irregular nests, accompanied by fibroblastic cells and fibrous stroma (a). Immunohistochemical (b-c) and fluorescent immunohistochemical (d-f) expression of CD73 and emmprin in epithelioid sarcoma specimen was examined. Both tumor cells and surrounding stromal cells were positive for CD73 (b). Membranous emmprin expression was observed only in tumor cells (c). Cytoplasmic CD73 (green) expression was observed in fibroblasts and in tumor cells (e). Membranous emmprin expression (red) was observed in tumor cells (f). Marger of figures (e) and (f). The green arrow, indicates fibroblasts expressing green florescence (CD73); The Yellow arrow, indicates tumor cells expressing yellow florescence (emmprin and CD73) (d). CD73 and emmprin were colocalized in tumor cells (e). Nuclei were stained with DAPI (blue). Expression of CD73 and emmprin was examined immunohistochemically in a total of ten tumors. All tumors show similar expression pattern (Additional file 6: Table S1)