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Fig. 1 | BMC Cancer

Fig. 1

From: The effects of photodynamic therapy on leukemia cells mediated by KillerRed, a genetically encoded fluorescent protein photosensitizer

Fig. 1

Expression and purification of KillerRed. a: Images of E. coli BL21(DE3) (left) and E. coli BL21(DE3) containing pKillerRed-B vector (right); b: SDS-PAGE gel of KillerRed expression. Lane M: molecular marker; lane 1: E. coli BL21(DE3) cells; lane 2: pKillerRed-B contained E. coli BL21(DE3) cells after IPTG induction for 4 h; lane 3: cells after IPTG induction for 10 h; lane 4: cells after IPTG induction for 24 h. c: SDS-PAGE gel of KillerRed purification. Lane M: molecular marker; lane 1: pKillerRed-B contained E. coli BL21(DE3) cells without IPTG induction; lane 2: cells after IPTG induction for 24 h; lane 3: supernatant of ultrasonication-treated cells; lane 4: purified KillerRed eluted from Q-Sepahrose Fast Flow column. d: FPLC elution profile of KillerRed. e: Western-blotting analysis of KillerRed. Lane 1–4: the same as (c)

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