Fig. 5

Knockdown of MAP 1B-LC1 repressed TGF-β1-induced EMT in A549 cells. a Western blotting analysis of MAP 1B-LC1 involved in EMT. A549 cells were transfected with MAP 1B-LC1 siRNA or NC siRNA, and stimulated with 5 ng/mL TGF-β1. b-d Quantifications of the expression of E-cadherin, vimentin and snail in A549 cells transfected with MAP 1B-LC1 siRNA or NC siRNA after TGF-β1 treatment. Mean ± standard deviation (error bars) of three separate experiments performed in triplicate. ^*P < 0.05. e Immunofluorescent analysis for EMT markers E-cadherin and F-actin polarization. E-cadherin (red), F-actin (green), and DAPI (blue) staining were shown, respectively. Bar, 100 μm. f Representative images of cell migration. The cells transfected with NC siRNA or hnRNP K siRNA were treated with TGF-β1, seeded in the inserts and incubated for 3 h. g The histograms showed the fold change of cell migration. Mean ± standard deviation (error bars) of three separate experiments performed in triplicate. ^*P < 0.05