Fig. 4

Expression of a CDglyTK fusion gene in gene-modified superficial inferior epigastric (SIEA) flaps. a Position of the pedicle, flap caudal, and flap cephalic in the free SIEA flap. b Reverse transcriptase polymerase chain reaction analysis of CD/TK fusion gene in SIEA flaps transfected with different recombinant lentivirus. c Protein expression of CD/TK double suicide gene by western blot in transfected flaps. Data was shown as the mean ± SD of three independent samples. LV-CDglyTK, flaps transfected with lentivirus-mediated CDglyTK gene; LV-GFP, flaps transfected with empty lentivirus; Control, non-transfected flaps