Fig. 2

Characterization of tumor-infiltrating leukocytes in EO771 and EONY tumors. 2 × 10⁵ EO771 cells, EONY#9 cells or EONY#17 cells were injected s.c. into the right flank of HLA-DRB1*0401tg mice (n = 10). Tumor-infiltrating leukocytes were isolated 20 days post cell implantation and analyzed by flow cytometry. a Percentage of CD8⁺ T cells, CD4⁺ T cells and CD11b⁺F4/80⁺ macrophages among CD45⁺ leukocytes is depicted for the different tumors. b The frequency of TAMs expressing M1-associated markers HLA-DR4 and iNOS or M2-associated marker CD206. c The corresponding surface expression levels (MFI) on the positive cells is presented. Error bars depict SEM and statistical analysis performed using One-way-ANOVA with Tukey’s multiple comparisons (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001)