Fig. 4

TfRCC tumor growth and mouse survival after treatment with dual mTORC1/mTORC2 versus selective mTORC1 inhibition. Nude mice bearing UOK120 or UOK146 tumor xenografts were treated with oral (PO) AZD8055, PO vehicle control, intraperitoneal (IP) sirolimus or IP vehicle control for a 3-week period. a, b Tumor growth curves showing average tumor volume over time for each treatment condition in UOK120 (a) and UOK146 (b) xenograft-bearing mice. AZD8055 significantly reduced tumor size compared to PO control (UOK120: p < 0.0001; UOK146: p < 0.0001) or sirolimus (UOK120: p = 0.004; UOK146: p = 0.0003). Growth curves are truncated at the time of the first mouse death for that condition. c, d Survival curves for xenograft-bearing mice. Sirolimus treatment showed no significant benefit on mouse survival compared to vehicle treated controls, while AZD8055 treatment extended survival compared to the PO control and sirolimus treatments in mice harboring UOK120 (c) or UOK146 (d) xenografts. Log-rank p-values: p = 0.021 for AZD8055 vs. PO control (UOK120); p = 0.076 for AZD8055 vs. sirolimus (UOK120); p = 0.815 for sirolimus vs. IP control (UOK120); p < 0.0001 for AZD8055 vs. PO control (UOK146); p < 0.0001 for AZD8055 vs. sirolimus (UOK146); p = 0.729 for sirolimus vs. IP control (UOK146)