Fig. 1

AMOG expression as assayed in a normal human brain and glioma tissue microarray. a Immunostaining of AMOG in the microarray containing both tumor-adjacent normal human brain and glioma tissues graded from I to IV. b Gray-scale image showing AMOG expression in all samples. c Samples from the tissue microarray were pooled in accordance with the WHO grading system. d Data were collected from all the samples arrayed in the slides for quantification. Values are expressed as means ± SEM. *p < 0.05, compared with normal brain tissues; ^#p < 0.05, compared with grade I glioma tissues; one-way ANOVA with Tukey’s post-hoc test. Samples of normal brain tissue (n = 18), pilocytic astrocytoma (WHO grade I, n = 23), WHO grade I-II (n = 19), diffuse astrocytoma (WHO grade II, n = 85), anaplastic astrocytoma (WHO grade III, n = 27), and glioblastoma (WHO grade IV, n = 31). e Representative AEC-based AMOG immunostained images in normal human brain tissues and different grades of human glioma tissues. Photographs were taken with a digital microscope. Scale bars, upper panels 500 μm, middle panels 200 μm, and lower panels 20 μm