Fig. 7
From: Response of neuroblastoma cells to RF currents as a function of the signal frequency
Immunoblot for Bax and flow cytometry analysis for cell cycle. a Representative blots of Bax at 12 h of sham-exposure (control), PD98059 inhibitor treatment, or CRET exposure in the presence of inhibitor. 100 μg protein/lane. β-actin was used as loading control. C: sham-exposed controls, PD: samples treated only with inhibitor PD98059, CRET+PD: exposed to CRET in the presence of the inhibitor. b Immunoblot densitometry for protein expression. The data, normalized over controls, are means ± SD values of the protein/β-actin ratios for the corresponding proteins at 12 h of treatment in 4 experimental repeats. NS: p ≥ 0.05; ANOVA and Bonferroni’s Multiple Comparison Test (c) Flow cytometry analysis for cell cycle phases and apoptosis after 24 h of sham-exposure, PD98059 treatment only or CRET-exposure in the presence of inhibitor. Percent of cells in cycle phases G0/G1, S or G2/M and of apoptotic cells SubG0/G1. Data are means ± SEM of at least 3 experimental repeats, normalized over the respective sham-exposed controls. *: 0.05 > p ≥ 0.01; ANOVA and Bonferroni’s Multiple Comparison Test