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Fig. 4 | BMC Cancer

Fig. 4

From: Ubiquitous expressed transcript promotes tumorigenesis by acting as a positive modulator of the polycomb repressive complex 2 in clear cell renal cell carcinoma

Fig. 4

UXT promotes EZH2-SUZ12 interaction. a UXT interacts with SUZ12, but not with EED, in EZH2-knockout cells. Cotransfection of EGFP-myc-UXT into 786-O or EZH2-knockout 786-O cells was performed, together with Flag-SUZ12, Flag-EED, or an empty control plasmid psi-Flag, respectively. 48 h after transfection, the whole cell lysate was extracted for co-immunoprecipitation with anti-Flag, followed by probing with anti-Myc. b EZH2 facilitates UXT binding with the other PRC2 components SUZ12 and EED. 293 T cells were co-transfected with the indicated combinations of plasmids. Cell lysates were precipitated with anti-Flag antibody and immunoblotted with anti-Myc antibody. c UXT binds to EZH2 and promotes the interaction between EZH2 and SUZ12, but has no effect on EZH2-EED interaction. 293 T cells were co-transfected with the indicated combinations of plasmids. Cell lysates were precipitated with anti-Flag antibody and immunoblotted with anti-Myc antibody

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