Fig. 2

UXT interacts with EZH2 in the nucleus. a Confocal microscopy shows the co-localization of EZH2 with UXT. 293 T cells were transfected with the EGFP–UXT and mCherry–EZH2 plasmids. The nuclei were stained with Hoechst 33258. b Western blot analysis confirmed the predominant expression of UXT and EZH2 protein in the nucleus of 293 T cells. c Endogenous EZH2 interacts with UXT in the nucleus. FLAG–UXT was transfected into 293 T cells. The nuclear and cytoplasmic fractions were isolated. Equal amounts of the fractions were immunoprecipitated with either control IgG or anti-FLAG antibody, followed by probing with an anti-EZH2 antibody