Fig. 2

Induction of IL-6 and STAT3/5 in cells resistant to copanlisib or duvelisib. Cytokine (a) and phospho-kinase (b) expression in Ly1-copanlisib resistant cells, HH-duvelisib resistant cells, and parental cells. Individual cytokines and phospho-kinases were spotted in duplicate; induced molecules are indicated and labeled (red squares). Positive control spots are located at the corners of the human cytokine array and phospho-kinase array. ELISA results indicate IL-6 protein levels in culture supernatants. p-STAT3/5 was measured by Western blot analysis and normalized to STAT3/5. Data represent mean values ± SEM of three independent experiments. Each experiment was performed with triplicate samples. P-values were determined by Student’s t-test