Fig. 1

Copanlisib-resistant B-cell lymphoma cell lines and a duvelisib-resistant T-cell lymphoma cell line were established by long term exposure to low concentrations of the respective agents. a Concentration-response curves for copanlisib and duvelisib in B- and T-cell lymphoma cell lines. Cells were treated with the indicated concentrations of copanlisib or duvelisib for 72 h, and subjected to CCK-8 assays. b The cell growth percentage was evaluated by comparing live cell numbers on days 0 and 3 after treatment with copanlisib (100 nM) or duvelisib (1 μM). Viable cells were counted after trypan blue staining. c, d Copanlisib- or duvelisib-resistant B- and T-cell lymphoma cell line were established by long-term exposure to the corresponding agents. Copanlisib and duvelisib resistance were confirmed using CCK-8 assays (c) and colony-forming assays (d). Data represent means ± standard deviation of triplicate values. P-values were determined by Student’s t-test