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Fig. 1 | BMC Cancer

Fig. 1

From: Generation of dual specific bivalent BiTEs (dbBIspecific T-cell engaging antibodies) for cellular immunotherapy

Fig. 1

Purification of a dbBiTE by size exclusion HPLC. a One hundred micrograms of a crude reaction mixture of DBCO-anti-CD3 antibody plus azido-PEG5-anti-CEA antibody were injected onto a Superdex 200 (1 × 30 cm) column, monitored at 214 nm (red) and 280 nm (blue) and eluted at a flow rate of 0.5 mL/min in PBS. The two peaks (peak 1 and 2) were collected and further analyzed. Based on calibration with authentic standards, peak 1 has a molecular mass of 300 kDa and peak 2, 150 kDa. b Peaks 1 and 2 from SE HPLC were run on non-reducing SDS gels to determine their molecular sizes compared to the standard IgG M5A. c The starting antibodies, anti-CEA M5A and anti-CD3 OKT3 were run as standards on SDS polyacrylamide gel electrophoresis alongside purified peak 1 dbBiTE

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