Fig. 3

Pathway activity by metaplastic subtype. a) Heatmap of ASSIGN pathway probabilities for metaplastic and triple negative breast cancer samples for experimentally-derived signatures and b) Heatmap of ASSIGN pathway probabilities for metaplastic and triple negative breast cancer samples for literature-derived signatures. TP53, PIK3CA, and “other” boxes indicate presence or absence of clinically significant mutations identified via Onco48 or Foundation Medicine panel. c) ASSIGN pathway activity scores by histological cancer subtype and d) ASSIGN pathway activity scores in all metaplastic and triple negative samples. *p < 0.05; **p < 0.01 via post-hoc Tukey test. Bars represent standard error of the mean. Mes: mesenchymal, spi: spindle cell, squ: squamous, spi + squ: mixed spindle cell and squamous, Anast. Inv.: Anastassiou multi-cancer invasiveness. Boq.: Boquest