Fig. 1

Influence of HER-2/HER-3 inhibition on cellular viability. Cellular viability of LS513, LS1034, and SW837 cells was determined 24 h (black curve), 48 h (red curve), and 72 h (blue curve) after treatment with increasing concentrations of trastuzumab (a), pertuzumab (b), T-DM1 (c), lapatinib (d), and afatinib (e). To assess the inhibitory effect on downstream signaling, cells were treated with increasing concentrations of the respective inhibitors for 24 h, stimulated with 100 ng/ml neuregulin for 10 min, and referred to Western blot analysis. All experiments were performed in triplicate, independently repeated three times. The respective P-values and the (estimated) EC₅₀ for all drugs and time points are listed in Additional file 5: Table S1