Fig. 3From: Lipid accumulation impairs natural killer cell cytotoxicity and tumor control in the postoperative periodLipid-laden NK cells show diminished expression of critical NK cell receptors. a Splenic single cell suspensions from untreated and surgery-treated mice were prepared, counted, stained with antibodies against various activating and inhibitory NK cell markers and analyzed by flow cytometry. Flow data are representative of three similar experiments where n = 4/group, p values as shown. b Immunoblot analysis measuring the expression of ITK, SLP-76, and PLCγ. β-actin was used as a loading control. Data is representative of 3 independent experimentsBack to article page