Fig. 1

Lipid accumulation in postoperative NK cells. a Splenic single cell suspensions from B16 tumor bearing C57Bl/6 (B6-B16) mice were prepared, counted, stained with NK markers (NK1.1⁺, CD3⁻) and BODIPY 495/593 and analyzed by flow cytometry. The Mean Fluorescent Intensity (MFI) of BODIPY⁺ NK cells is shown from surgery-treated and untreated mice. b Fluorescent microscopy imaging of purified NK cells from surgery-treated and untreated mice stained with BODIPY 495/503. c MFI of BODIPY⁺ NK cells (CD122⁺, CD3⁻) is shown from surgery-treated and untreated mice in CT26 tumor bearing BALB/c mice. MFI of (d) BODIPY⁺ macrophages (F4/80⁺, CD11b⁺) and (e) conventional dendritic cells (CD11c+, mPDCA1⁻) is shown from surgery-treated and untreated B6-B16 mice. Pooled data are displayed from three similar experiments, p values as shown (n.s., no significance)