Table 1 Primary antibodies used for western blotting analysis in this study. Antibodies dilutions and the diluent in which they were prepared are also specified. With the exception of Anti-Surfactant protein D antibody [12G5] (Abcam, Ireland) and mouse anti-human fibronectin N-terminal monoclonal antibody (Millipore Merck, Ireland), all antibodies were purchased from Cell Signaling Technology Inc. (Brennan & Company, Ireland)
Antibody | Dilution | Diluent |
|---|---|---|
BCL-2 (D55G8) Rabbit mAb (Human Specific) | 1:1000 | 5% BSA in TBS-T 1× |
Phospho-MDM2 (Ser166) Antibody | 1:1000 | 5% BSA in TBS-T 1× |
Phospho-p53 (Ser15) (16G8) Mouse mAb | 1:500 | 5% BSA in TBS-T 1× |
Akt (pan) (C67E7) Rabbit mAb | 1:1000 | 5% BSA in TBS-T 1× |
Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb | 1:2000 | 5% BSA in TBS-T 1× |
Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody | 1:1000 | 5% BSA in TBS-T 1× |
Mouse anti-human fibronectin N-terminal monoclonal antibody | 1:1000 | 5% BSA in TBS-T 1× |
Anti-Surfactant protein D antibody [12G5] | 1:20000 | 5% BSA in TBS-T 1× |
phospho-mTOR (Ser2448) (D9C2) XP® Rabbit mAb | 1:1000 | 5% BSA in TBS-T 1× |
c-IAP1 (D5G9) Rabbit mAb | 1:1000 | 5% BSA in TBS-T 1× |
c-IAP2 (58C7) Rabbit mAb | 1:1000 | 5% BSA in TBS-T 1× |
MCL-1 (D35A5) Rabbit mAb | 1:1000 | 5% BSA in TBS-T 1× |
MDR1/ABCB1 (D3H1Q) Rabbit mAb | 1:1000 | 5% BSA in TBS-T 1× |
Vimentin (D21H3) XP® Rabbit mAb | 1:1000 | 5% BSA in TBS-T 1× |
Phospho-SMAD2 (Ser465/467) (138D4) Rabbit mAb | 1:1000 | 5% BSA in TBS-T 1× |
β-Actin Antibody | 1:1000 | 5% BSA in TBS-T 1× |
α–Tubulin Antibody | 1:1000 | 5% BSA in TBS-T 1× |
Caspase-3 Antibody | 1:1000 | 5% non-fat dry milk in TBS-T 1× |
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb | 1:1000 | 5% non-fat dry milk in TBS-T 1× |
PARP (46D11) Rabbit mAb | 1:1000 | 5% non-fat dry milk in TBS-T 1× |
MRP1/ABCC1 (D708N) Rabbit mAb | 1:1000 | 5% non-fat dry milk in TBS-T 1× |
Bcl-xl (54H6) Rabbit mAb | 1:1000 | 5% non-fat dry milk in TBS-T 1× |
Survivin (6E4) Mouse mAb | 1:1000 | 5% non-fat dry milk in TBS-T 1× |
E-Cadherin (4A2) Mouse mAb | 1:1000 | 5% non-fat dry milk in TBS-T 1× |
GADPH (D16H11) XP® Rabbit mAb | 1:1000 | 5% non-fat dry milk in TBS-T 1× |