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Fig. 6 | BMC Cancer

Fig. 6

From: ALI multilayered co-cultures mimic biochemical mechanisms of the cancer cell-fibroblast cross-talk involved in NSCLC MultiDrug Resistance

Fig. 6

Investigation of cancer cell-fibroblast cross-talk through physical and chemical stimuli. a Representative LSCM image of ALI multilayered co-cultures. Cells were stained for F-actin (in red) and Z-stack images of the cultures were reconstructed and are shown in side view mode. The right hand-side of (a) shows the red fluorescence intensity profile of the selected region of interest from a (left hand-side). An F-actin filament extending from the apical to the basolateral side is visible. Objective lens: 63×. b Concentration of EGF, free-active TGF-β1 and total TGF-β1 detected in the supernatants harvested from the apical (top graph) and basolateral (bottom graph) compartments of the Transwell™ supports when A549 cells were cultured as ALI multilayered mono- or co-cultures. p values < 0.05 indicate significant differences (one-way ANOVA followed by Dunnett post-test)

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