Table 2 RNAseq comparing no treatment controls versus 18 h treatment with: a.) control versus MP1 at 500 nM, b.) control versus TEM alone at 1 μM, and c) control versus combination of MP1 plus TEM. All treatments were performed in triplicate and fold change calculated from the ratio of treatment over control
a.) | ||||||||
Gene | Change | p-value | Cntrl#1 | Cntrl#2 | Cntrl #3 | MP1#1 | MP1#2 | MP1#3 |
MYCN | −2.0 | 6.09 E-179 | 2125 | 2166 | 2202 | 1092 | 1087 | 1096 |
 MCL-1 | −1.18 | 3.38E-09 | 318 | 309 | 315 | 265 | 268 | 267 |
 LCII | + 1.8 | 4.08 E-59 | 67.5 | 70.7 | 76.0 | 135 | 128.9 | 131.4 |
 LCI | + 1.6 | 1.38 E-03 | 2.68 | 3.8 | 2.67 | 4.23 | 6.13 | 4.27 |
 PARP1 | −1.5 | 8.56 E-52 | 699 | 711.6 | 689.6 | 451.8 | 466.3 | 463.9 |
 BRD-4 | + 1.07 | 0.15 | 77.3 | 75.5 | 75.2 | 84.5 | 82.2 | 77.4 |
 Bcl-2 | 1.76 | 1.09 E-35 | 33.7 | 34.6 | 34.1 | 64.0 | 50.5 | 56.9 |
 BAX | −1.39 | 8.51 E-12 | 46.1 | 47.6 | 43.4 | 35.0 | 31.5 | 32.0 |
b.) | ||||||||
Gene | Change | p-value | Cntrl#1 | Cntrl #2 | Cntrl #3 | TEM#1 | TEM#2 | TEM#3 |
 MYCN | 1.0 | 0.35 | 2125 | 2166 | 2202 | 2264 | 2140 | 2232 |
 MCL-1 | −1.12 | 2.48E-05 | 318 | 309 | 315 | 281 | 282 | 276 |
 LCII | + 1.3 | 1.44 E-10 | 67.5 | 70.7 | 76.0 | 92.7 | 92.5 | 89.7 |
 LCI | + 1.5 | 0.004 | 2.68 | 3.8 | 2.67 | 5.1 | 4.4 | 4.4 |
 PARP1 | −1.2 | 1.01E-08 | 699 | 712 | 690 | 580 | 617 | 599 |
 BRD-4 | −1.06 | 0.20 | 77.3 | 75.5 | 75.2 | 70.4 | 71.4 | 72.7 |
 Bcl-2 | 1.45 | 9.03 E-16 | 33.7 | 34.6 | 34.1 | 49.2 | 52.0 | 47.7 |
 BAX | −1.14 | 0.007 | 46.1 | 47.6 | 43.4 | 42.7 | 37.3 | 40.6 |
c.) | ||||||||
Gene | Change | p-value | Cntrl#1 | Cntrl #2 | Cntrl #3 | MP1 + TEM #1 | MP1 + TEM #2 | MP1 + TEM #3 |
 MYCN | −1.6 | 9.1E-89 | 2125 | 2166 | 2202 | 1338 | 1347 | 1356 |
 MCL-1 | −1.2 | 1.29E-08 | 318 | 309 | 315 | 266 | 271 | 269 |
 LCII | + 1.7 | 2.28 E-42 | 67.5 | 70.7 | 76.0 | 113 | 125 | 120 |
 LCI | + 2.5 | 2.11E11 | 2.68 | 3.8 | 2.67 | 8.19 | 8.21 | 6.5 |
 PARP1 | −1.6 | 6.48E-64 | 699 | 712 | 690 | 432 | 440 | 447 |
 BRD-4 | −1.09 | 0.078 | 77.3 | 75.5 | 75.2 | 78.6 | 62.5 | 68.8 |
 Bcl-2 | 1.94 | 5.9 E-51 | 33.7 | 34.6 | 34.1 | 66.2 | 63.8 | 68.5 |
 BAX | −1.29 | 8.67 E-08 | 46.1 | 47.6 | 43.4 | 34.3 | 36.5 | 35.8 |