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Fig. 6 | BMC Cancer

Fig. 6

From: CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer

Fig. 6

CircRNA CBL.11 regulates the cell proliferation through miR-6778-5p and YWHAE. a The levels of YWHAE in CRC cells at 24 h after carbon ion irradiation (2Gy) were measured by real-time PCR. GAPDH was used as a control. b-c CircRNA CBL.11 and CBL were detected using real-time PCR after siRNA transfection. si-circRNA CBL.11 only knocked down circular transcripts but it is difficult to affect the expression of linear species. However, si-CBL only knocked down its linear transcript, it did not affect the circular transcript. GAPDH was used as a control. d MiR-6778-5p level was analyzed by real-time PCR after transfection with si-circRNA CBL.11 and NC siRNA, which was normalized to the U6 expression level. e-f HCT116 cells were transfected with siRNA and/or inhibitor, and the expression of YWHAE was measured by real-time PCR and western blot. The horizontal line indicates the same processing. g Viability of HCT116 cells was measured using CCK-8 assay at 24 h after transfection with siRNA and/or miRNA inhibitor. The horizontal line indicates the same processing. h Analysis of DNA synthesis of HCT116 cells transfected with siRNA and/or miRNA inhibitor by EdU assay. Bars, 100 μm. Data are represented as mean ± SD. *P < 0.05, **P < 0.01 versus the control group

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