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Fig. 3 | BMC Cancer

Fig. 3

From: CircRNA CBL.11 suppresses cell proliferation by sponging miR-6778-5p in colorectal cancer

Fig. 3

MiR-6778-5p is involved in the regulation of YWHAE expression. a A Venn diagram showing the overlap among the predicted targets of miR-6778-5p. b Analysis of YWHAE 3′-UTR binding site for miR-6778-5p by RNAhybrid tools. Filtering parameters were as follows: energy threshold<− 20; approximate p-value 3utr_human. c The levels of YWHAE in CRC cells at 24 h after carbon ion irradiation (2Gy) were measured by real-time PCR. GAPDH was used as a normalized control. d The level of YWHAE in HCT116 cells at 48 h after carbon ion irradiation was measured by means of western blot analysis, with α-tubulin serving as the loading control. e-f HCT116 cells were transfected with a miR-6778-5p mimic (mimic-6778)/ miR-6778-5p inhibitor (inhibitor-6778) or the control mimic (mimic-NC)/ (inhibitor-NC); the expression of YWHAE were detected using real-time PCR and western blot. g Dual-Luciferase reporter about YWHAE 3′-UTR binding site for miR-6778-5p. h HCT116 cells were co-transfected with miR-6778-5p mimic or control mimic and luciferase reporter containing YWHAE 3′-UTR or its mutant construct. The luciferase activity was detected 24 h after transfection. Data are represented as mean ± SD. *P < 0.05, **P < 0.01 versus the control group

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