Fig. 2

Graphic diagram showing the protein domains and distribution of significant genetic mutations identified in TP53 and FAT4. (b) p53 protein with 393 amino acids and consists of transactivation, proline-rich, DNA binding, oligomerization and regulation domains. Partial electropherograms of three representative mutations resulted in amino acid alterations (Y220S, R249S and P250R) identified in TP53 are shown. (b) FAT4 protein with 4981 amino acids and consists of 34 cadherin domains, 5 EGF-like domains and 2 laminin G-like domains. Partial electropherograms showing two representative non-synonymous mutations (P4972S and D2826N) identified in FAT4. The locations of the genetic variants are indicated by red arrows for synonymous mutation and black bars for non-synonymous mutation. The position of mutation is bolded and marked with asterisk in reference sequence for TP53 (NM_001125115) and FAT4 (NM_024582). (Ref: reference sequence; Mut: mutated sequence)