Fig. 5
From: G-protein coupled receptor 64 (GPR64) acts as a tumor suppressor in endometrial cancer
The reduction of Cx43 levels by GPR64 loss through AMPK activation in Ishikawa cells. a The Western blot analysis of phospho-AMPKThr172, Total AMPK, and Cx43 proteins in Ishikawa cells transfected with NT siRNA or GPR64 siRNA on day 1 and day 6. Actin was used as sample-loading control. b Quantification of western blot analysis of Cx43. c The expression of Cx43 gene during in vitro culture after GPR64 siRNA transfection. Cx43 was significantly decreased on day 3 and day 6 in cells transfected with GPR64 siRNA. The results represent the mean ± SEM. *, p < 0.05; and **, p < 0.01