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Fig. 7 | BMC Cancer

Fig. 7

From: Identification of a novel S6K1 inhibitor, rosmarinic acid methyl ester, for treating cisplatin-resistant cervical cancer

Fig. 7

RAME enhances the effects of cisplatin in cervical cancer cells. a Immunoblotting analysis of SiHa cells treated with or without cisplatin (5 μM) and RAME (80 μM) for 24 h. b Immunoblotting analysis of GFP-LC3B-expressing SiHa cells treated with or without cisplatin (5 μM) and RAME (80 μM) for 24 h. c Fluorescent imaging of GFP-LC3B-expressing SiHa cells treated with or without cisplatin (5 μM) and RAME (80 μM) for 24 h. d The mRNA levels of autophagy-related genes in SiHa cells treated with or without cisplatin (5 μM) and RAME (80 μM) for 24 h. e The mRNA levels of apoptosis, DNA repair, and cell cycle arrest marker genes in SiHa cells treated with or without cisplatin (5 μM) and RAME (80 μM) for 24 h. f Clonogenic assay of HeLa and SiHa cells treated with or without cisplatin (1 μM) and RAME (40 μM) for 10~20 days. g IC50 values of cisplatin in SiHa cells treated with or without RAME (80 μM) for 24 h. Error bars correspond to mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001; unpaired t test

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