Fig. 5

RAME induces apoptosis in cervical cancer cells. a and b Immunoblotting analysis of HeLa (a) and SiHa (b) cells treated with RAME (40 or 80 μM) for 24 h. (c) Immunoblotting analysis of HeLa cells transfected with siRNA targeting S6K1 and treated with RAME (80 μM) for 24 h. d and e The mRNA levels of apoptosis, DNA repair, and cell cycle arrest marker genes in HeLa (d) and SiHa (e) cells treated with RAME (40 or 80 μM) for 24 h. f and g Cell viability of HeLa (f) and SiHa (g) cells treated with RAME (40 or 80 μM) for 24 and 48 h. Error bars correspond to mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001; unpaired t test