Fig. 4

RAME induces autophagy in cervical cancer cells. a and b Immunoblotting analysis of GFP-LC3B-expressing HeLa (a) and SiHa (b) cells treated with RAME (40 or 80 μM) for 24 h. c Immunoblotting analysis of HeLa cells transfected with siRNA targeting S6K1 and treated with RAME (80 μM) for 24 h. (d and e) Fluorescent imaging of GFP-LC3B-expressing HeLa (d) and SiHa (e) cells treated with RAME (80 μM) for 24 h. f and g The mRNA levels of autophagy-related genes in HeLa (f) and SiHa (g) cells treated with RAME (40 or 80 μM) for 24 h. Error bars correspond to mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001; unpaired t test