Fig. 2

RAME, not RA, inhibits kinase activity of S6K1 in vitro and in vivo. a Structures of RAME and RA. b and c The in vitro kinase assay with RAME (b) or RA (c) was performed in a dose dependent manner using recombinant GST-S6, active S6K1, and cold-ATP. d Quantitative graph of (b and c). e Immunoblotting analysis of HeLa (left) and SiHa (right) cells treated with RAME (80 μM) or RA (80 μM) for 24 h