Fig. 2

MiR-330-5p regulates the expression of extracellular MMP1 protein expression. a MMP1 protein expression in conditioned media was increased in the OE33 miRZIP-330-5p SC compared to the miRZIP-VC SC. The blot is representative of n = 3 independent experiments. Statistical analysis was performed using densitometry data and a one-tailed unpaired t-test; * p < 0.05. b MMP1 protein expression in conditioned media was increased in the OE33 miRZIP-330-5p HC compared to the miRZIP-VC HC. The expression of MMP7 protein was not increased by miR-330-5p silencing. The blot is representative of n = 3 independent experiments. Statistical analysis was performed using densitometry data and the unpaired t-test; * p < 0.05; ns, not significant. c In the OE33 cell line the transient overexpression of miR-330 significantly decreased the expression of MMP1 protein in the 24 h conditioned media (48 h post-transfection) compared to the miR-VC. Blots represent n = 3 independent experiments. Statistical analysis was performed using densitometry data and the paired t-test; * p < 0.05; ns, not significant