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Table 3 Primers (5′ → 3′) and cycling conditions used in MSP

From: Regulation of human chorionic gonadotropin beta subunit expression in ovarian cancer

Primer NameForward primerReverse primerAnnealingElongation
CGB1–2_ Mgaaattaagttcgaagtcgccctatcaaccataacgatcg30 s, 51 °C30 s, 72 °C
CGB1–2_ NMgtagaaattaagtttgaagttgtcctatcaaccataacaatca30 s, 47 °C30 s, 72 °C
CGB3–9_ Mtgtttagtttgatggtatcgcatacccgaaacgatcccc30 s, 58 °C30 s, 72 °C
CGB3–9_NMaattgtttagtttgatggtattgtaaaatacccaaaacaatcccc30 s, 55 °C30 s, 72 °C
DAPKattgggaaggttaaggyggagggaaatttggtccccaaacraaacaatccccaaaaccacattccta30 s, 59 °C60 s, 72 °C
  1. M methylated variant, NM nonmethylated variant