Table 3 Primers (5′ → 3′) and cycling conditions used in MSP

CGB1–2_ M

gaaattaagttcgaagtcgc

cctatcaaccataacgatcg

30 s, 51 °C

30 s, 72 °C

CGB1–2_ NM

gtagaaattaagtttgaagttgt

cctatcaaccataacaatca

30 s, 47 °C

30 s, 72 °C

CGB3–9_ M

tgtttagtttgatggtatcgc

atacccgaaacgatcccc

30 s, 58 °C

30 s, 72 °C

CGB3–9_NM

aattgtttagtttgatggtattgt

aaaatacccaaaacaatcccc

30 s, 55 °C

30 s, 72 °C

DAPK

attgggaaggttaaggyggagggaaatttggt

ccccaaacraaacaatccccaaaaccacattccta

30 s, 59 °C

60 s, 72 °C