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Table 2 Primers (5′ → 3′) and hydrolysis probes for RT-qPCR with the use of SYBR Green and TaqMan assays

From: Regulation of human chorionic gonadotropin beta subunit expression in ovarian cancer

 GeneForward primerReverse primerHydrolysis probe
SYBR GreenSP1tgaaggaaggggctcgggggccaggggcaaagtgcccacan/a
SP3caggatgtggtaaagtctatggcttctcacctgtatgtgttcttcn/a
TFAP2Agctgcctcaccagctgtcggacagggacacggggcctttctn/a
HPRTtgaagagctattgtaatgaccagtcaaatccaacaaagtctggcn/a
TaqManCGB1–2 aggtccgctgactcyggc bcagcagcagcccctttgac6FAM-tcactccctgtctcactcccccacg-BBQ
CGB3–9 agtgtcsagctcacyccagcatccta bagcagcccctggaacatct6FAM-ccgaggtytaaagccaggtacacsaggc-BBQ
HPRTHuman HPRT Gene Assay Cat. No. 05 046 157 001 (Roche Diagnostics, Mannheim, Germany)
  1. a CGB3–9 and CGB1–2 hydrolysis probes were designed and manufactured by Tib Molbiol (Berlin, Germany); b degenerated base code: S-G/C; Y-C/T