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Fig. 3 | BMC Cancer

Fig. 3

From: Genipin induces mitochondrial dysfunction and apoptosis via downregulation of Stat3/mcl-1 pathway in gastric cancer

Fig. 3

Treatment of Genipin affects apoptosis by downregulating phosphorylated Stat3. a, b AGS cells were treated with 150 μM Genipin for 24 h. Cell lysates were hybridized using the Proteome Profiler Phospho-Kinase Array kit (a). The graph represents quantification of mean pixel density using Image J (1.5 version) program (b). c Protein levels of Stat3, phospho-Stat3, JAK2, and phospho-JAK2 were confirmed by immunoblotting in AGS cells treated with Genipin for 24 h. β-Actin was used as a loading control. d AGS cells treated with or without 150 μM Genipin were immunostained with anti-p-Stat3 (red) and Mcl-1 (green). Images were captured using a confocal microscope. e AGS cells were transfected with Stat3 siRNA (siStat3). After incubation, transfected cells were treated with 150 μM Genipin for 24 h. Cell lysates were detected by western blotting with the indicated antibodies. f Cells were treated with 150 μM Genipin for 24 h following pre-treatment of 10 μM ruxolitinib for 1 h. Cell lysates were evaluated by western blotting with the indicated antibodies. g AGS cells were treated with 150 μM Genipin for 24 h following pre-treatment with 50 nM IL-6 for 30 min. Protein levels of Stat3, phosphorylated Stat3, Mcl-1, and cleaved PARP were assessed by immunoblotting

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