Fig. 4
From: microRNA-21 promotes breast cancer proliferation and metastasis by targeting LZTFL1
LZTFL1 knockdown reverses miR-21 inhibitor-induced suppression of breast cancer proliferation and migration. a The original parental Hs578T cells (0 h) was labeled with eFluor™ 670 dye represented as the initial labeling control group. Then the labeled cells were treated with miR-21 inhibitor, LZTFL1 siRNA alone, or combined for 48 h represented as dividing shifted populations. b The mean fluorescence value of each group were caculated. c Wound healing assays in Hs578T cells treated with miR-21 inhibitor, LZTFL1 siRNA alone, or combined (40× magnification). d Transwell assay in Hs578T cells following the treatments indicated above (100× magnification). e Relative wound closure was calculated for the data in (c), and the experiments were performed in triplicate. f The relative percentage of migrated cells was determined for the data in (d), and the experiments were performed in triplicate. (*p < 0.05, **p < 0.01, ***p < 0.001)