Fig. 2
From: microRNA-21 promotes breast cancer proliferation and metastasis by targeting LZTFL1
Inhibition of miR-21 reduced colony formation and cell migration in breast cancer. a Colony-formation assay for Hs578T and MDA-MB-231 cells transfected with miR-21 inhibitor or non-targeted control (NT). The number of colonies was determined after 14 days. b Fold changes in colony number in the absence and presence of inhibitor were compared in three independent experiments. c Wound healing assay in Hs578T and MDA-MB-231 cells transfected with miR-21 inhibitor (40× magnification). d The relative wound closure was calculated after the experiments were performed in triplicate. e Transwell assay in Hs578T and MDA-MB-231 cells transfected with miR-21 inhibitor (100× magnification). f The relative migration of cells was determined after the experiments were performed in triplicate (*p < 0.05, **p < 0.01, ***p < 0.001)