Fig. 6
From: Metformin reverses mesenchymal phenotype of primary breast cancer cells through STAT3/NF-κB pathways
Metformin inhibits IL-6-induced cell proliferation and migration. a MBCDF and MBCD17 primary breast cancer cell lines were seeded at 15000 cells/cm2 in a 24-well plate and incubated under the absence (control) or presence of 10 ng/mL IL-6, 10 mM MTF or the combination of IL-6 and MTF. Cell proliferation was studied at days 0, 1, 3, and 5 by MTT. Data represent the mean ± SEM of three independent experiments performed in triplicate incubations. *P < 0.05, **P < 0.001. b Migration assays were tested using Boyden chambers. MBCDF and MBCD17 were seeded at 30000 cells/transwell in triplicate in the upper chamber. In the bottom chamber the same conditions were maintained as in Fig. 5a. Cells were allowed to migrate for 6 h. Migrating cells were fixed and stained with crystal violet. Data are presented as the mean ± SEM of three independent experiments. **P < 0.001