Fig. 3
From: Metformin reverses mesenchymal phenotype of primary breast cancer cells through STAT3/NF-κB pathways
Primary epithelial breast cancer cells undergo IL-6-induced EMT through STAT3 and NF-κB activation. Primary breast cancer cells with epithelial phenotype (MBCDF and MBCD17) were treated with 40 ng/mL of IL-6 during 0, 1 and 2 days. a Induction of EMT was analyzed by assessing the expression of E-cadherin, Vimentin, and SNAIL by Western blots. b The activation of STAT3 was measured by phosphorylation of STAT3 on tyrosine 705 using a phospho-specific anti-pSTAT3 Y705 antibody. c Activation of NF-κB was assessed by analyzing phosphorylation of NF-κB/p65 on serine 536 employing a phospho-specific anti- pNF-κB S536 antibody. Actin was used as loading control in all cases