Fig. 4

PTTG3P directly interacts with miR-383 in HCC cells. a The alignment between PTTG3P and miR-383. b Luciferase reporter assay was performed to detect the effect of miR-383 on luciferase intensity controlled by PTTG3P or mutant PTTG3P. c qPCR was used to analyze miR-383 expression in HepG2 and Huh-7 cells transfected with PTTG3P, or PTTG3P siRNA or controls. d miR-383 expression was analyzed by qPCR in 50 paired HCC tissues and adjacent non-tumor tissues. In addition, there was a negative correlation between miR-383 and PTTG3P expression in HCC. e RNA pull-down assay was performed to determine the interaction between PTTG3P and miR-383 in HCC cells. The biotinylated miR-383 or mutant miR-383 was transfected into HCC cells, and PTTG3P expression was analyzed by qPCR. f Anti-Ago2 RIP was used to detect the miR-383 and PTTG3P enrichment in immunoprecipitates in HCC cells. IgG was used as a negative control. The results were shown as mean ± SD from three independent experiments and one of the representative results was shown. Each experiment was performed in triplicate. *P < 0.05. **P < 0.01