Fig. 2

Knockdown of PTTG3P suppresses cell proliferation and promotes cell apoptosis. a qPCR analysis of PTTG3P expression in PTTG3P siRNA-transfected HepG2 and Huh-7 cells. b CCK-8 assay was used to detect the viability of PTTG3P siRNA in HepG2 and Huh-7 cells. c Colony formation assay was performed to detect the colony-forming ability of PTTG3P siRNA in HepG2 and Huh-7 cells. Colonies were counted and captured. d Flow cytometry was performed to investigate the cell cycle distribution of HepG2 and Huh-7 cells. The color code represented the G1 stage, S stage and G2 stage, respectively (Left to right). e Flow cytometry was performed to detect the apoptosis of HepG2 and Huh-7 cells. LR, early apoptotic cells; UR, late apoptotic cells or dead cells. The results were shown as mean ± SD from three independent experiments and one of the representative results was shown. Each experiment was performed in triplicate. *P < 0.05. **P < 0.01