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Fig. 3 | BMC Cancer

Fig. 3

From: Increased PHGDH expression promotes aberrant melanin accumulation

Fig. 3

Increased PHGDH expression in melanocytes drives melanin accumulation in early anagen hair follicles and increases melanocyte abundance. a PHGDHtetO mice were crossed to Dct-rtTA mice to drive increased PHGDH expression solely in melanocytes. Quantitation of the percent of early anagen hair follicles (HFs) containing any melanin granules in skin from Dct-rtTA mice with the indicated PHGDHtetO genotype exposed to doxycycline for 30 days prior to hair follicle synchronization as described in Fig. 2. Data shown represent the % observed when analyzing 50 HFs per mouse from 3 mice of each genotype. b Quantitation of the percent of early anagen hair follicles (HFs) with three or more melanin granules in in skin from mice described in (a) exposed to doxycycline for 30 days prior to hair follicle synchronization. Data shown represent the % observed when analyzing 50 HFs per mouse from 3 mice of each genotype. c Quantitation of the percent of early anagen hair follicles (HFs) containing any melanin granules in skin from mice described in (a) exposed to doxycycline for 2 days prior to hair follicle synchronization. Data shown represent the % observed when analyzing 50 HFs per mouse from 3 mice of each genotype. d Quantitation of the percent of early anagen hair follicles (HFs) with three or more melanin granules in skin from mice described in (a) exposed to doxycycline for 2 days prior to hair follicle synchronization. Data shown represent the % observed when analyzing 50 HFs per mouse from 3 mice of each genotype. The increase in hair follicles with melanin granules shown in (a-d) is statistically significant with p-values derived from two-tailed Fisher’s exact test. e PHGDHtetO; Dct-rtTA mice were crossed to H2B-GFPtetO mice such that melanocytes would express both PHGDH and GFP. qPCR to assess tyrosinase expression (a melanocyte-specific enzyme) in GFP- and GFP+ cells isolated from PHGDHtetO;Dct-rtTA; H2B-GFPtetO mice exposed to doxycycline for 30 days prior to hair follicle synchronization. Data shown represent the mean (+/− SD). The increase in tyrosinase expression is significant with p-values derived from an unpaired Student’s t test. f Mice described in (e) with or without a PHGDHtetO allele we exposed to doxycycline for 30-days prior to hair follicle synchronization and subsequent collection of early anagen skin samples. Cells isolated from skin were analyzed by flow cytometry to assess GFP+ melanocyte abundance. Data shown represent the mean (+/− SD). The increase in GFP+ melanocytes from PHGDHtetO/+ mice is statistically significant with p-values derived from an unpaired Student’s t test

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