Fig. 3

The miR-1275-JAZF1 link regulates vimentin and E-cadherin expression in GC cells. a Western blot analysis of vimentin and E-cadherin expression in MGC803 cells transfected with miR-1275 or miR-Ctrl and in SGC7901 cells transfected with anti-miR-1275 or anti-miR-Ctrl. Western blot analysis of E-cadherin and vimentin expression in MGC803 cells transfected with siJAZF1 or siNC. b GC cells were transfected with a JAZF1 expression vector or JAZF1 siRNA to restore or inhibit, respectively, the expression of E-cadherin and vimentin. c Schematic of the structure of the vimentin promoter. The numbers-1416 and + 72 depict the locations of the PCR primers used for vimentin promoter amplification. d Silencing JAZF1 decreased vimentin promoter activity. HEK293T cells were co-transfected with the vimentin-Luc reporter plasmid or pGL3 basic plasmid and Renilla with either siJAZF1 or siNC. Luciferase values were normalized to Renilla levels and expressed as the fold change over siNC. e ChIP analysis of JAZF1 at the vimentin promoter. Anti-JAZF1 or control IgG was used to immunoprecipitate the DNA-protein complex in MGC803 cells. Data regarding DNA precipitated by either anti-JAZF1 or control IgG are depicted as percentages of the total genomic DNA input. Numerical data are presented as the mean ± SD. **P < 0.01, ***P < 0.001