Fig. 1

Immunohistochemistrical staining of NR2F1and Ki-67 in SACC tissues and TUNEL staining. (a) Comparison of NR2F1, Ki-67 and TUNEL expression in the same areas of SACC between metastasis and no metastasis. A1-C1 showed the expression of NR2F1 was 15–20%,and Ki-67 was 0–1% (D1-F1) and TUNEL was negative expressed in G1-I1. In A2-C2, NR2F1 had no expression, while the expression of Ki-67 was a percentage of 5–10% in D2-F2. And TUNEL was positive in G2-I2. Scale bar = 100 μm, SP × 100; Scale bar = 20 μm, SP × 200; Scale bar = 20 μm, SP × 400, respectively. (b) The proportion of the positive cells of NR2F1, Ki-67, and TUNEL were calculated, respectively. Student’s paired t test was used to analyze the differences between the cases of primary tumors with metastasis and without metastasis. *P<0.05, **P<0.01