Fig. 3

Effects of cisplatin (CDDP) alone or with caffeine (caf), citrate (CA), or caffeine citrate (cafCA) on proliferation of human (HOS) and human fibrosarcoma cells (HT1080). a Representative immunofluorescence microscopic findings of expression of the EdU-positive proliferating cells, including overlay with Hoechst-stained micrographs. Scale bar = 50 μm. The number in the Overlay panels is the rate of EdU-positive cells. b Effects of the various treatments on the numbers of EdU-positive proliferating cells. Data are mean percentages of EdU-positive proliferating cells in five microscopic fields with standard deviations

EdU, 5-ethynyl-2-deoxyuridine.