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Fig. 2 | BMC Cancer

Fig. 2

From: Epithelial-mesenchymal transition markers screened in a cell-based model and validated in lung adenocarcinoma

Fig. 2

CAF-CM induces expression of mesenchymal markers in A549 cell. a Immunostaining of E-cadherin (green), vimentin (red) and DAPI (blue) of CAF treated and control cells. Beginning at 3 h, CAF treated cells showed increased vimentin and decreased E-cadherin expression compared to controls. b RT-qPCR of mRNA levels of EMT marker genes in A549 cells with CAF conditioned medium or control treatment at 3, 24 and 72 h. Error bars are SD. *, p < 0.05; **, p < 0.01; t test

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