Fig. 3

Metastatic capacity of cell lines and an equal mix of all cell lines. a Absolute count of micrometastases derived from orthotopic primary tumors, 34 days after injection of tumor cells. b-c Metastasis growth induced 35 days after intravenous injection of tumor cells: (b) lung area occupied by metastases, and (c) lung weight, an indirect measurement of lung tumors. Significant differences were determined using unpaired t-test with Welch’s correction. Asterisks indicate significant differences when P-values are < 0.05 (*), < 0.01 (**), and < 0.001 (***). d-e Representative fluorescent images (scale bar = 50 μm) of four independent sections of lung metastasis formed by MDA-MB-231 parental and GFP C3, mKO E10 and Sapphire clonal cell lines (mix)