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Fig. 4 | BMC Cancer

Fig. 4

From: APC2 is critical for ovarian WNT signalling control, fertility and tumour suppression

Fig. 4

Identifying molecular mechanisms associated with subfertility in APC2-deficient ovaries. a Representative photomicrographs of H&E and β-catenin immunohistochemical staining of atretic follicles showing high expression of β-catenin in granulosa cells of atretic follicles in Apc2−/− vs. Apc2+/+ ovaries. Bar = 100 μm; insets magnified 2X. b Expression levels of a subset of WNT-target genes compared between Apc2+/+ and Apc2−/− ovarian extracts by qRT-PCR. Expression levels of five out of eight genes analysed were significantly elevated in Apc2−/− ovaries (mean ± 95% confidence intervals; n = 4; *P < 0.05, **P < 0.01, determined from confidence intervals) [45]. c, d Relative expression levels of a panel of (c) Fox transcription factors, and (d) FOX downstream target genes in Apc2+/+ and Apc2−/− 10-week-old ovaries (mean ± 95% confidence intervals; n = 4 for all measurements except for Fasl and Foxo1 in Apc2+/+ where n = 3; *P < 0.05, **P < 0.01, determined from confidence intervals) [45]. e, f, g Representative photomicrographs of (e) PTEN, (f) p-AKT (ser-473), and (g) p-FOXO1,3,4 immunostaining in Apc2+/+ and Apc2−/− ovarian follicles. Bars = 200 μm; insets are magnified 2X

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