Fig. 1

APC2 loss causes subfertility in adult female mice. a Mating efficiency of Apc2 experimental genotypes as a function of time recorded in days between pairing the mice and delivering the first litter. N = 4 breeding cages each with one Apc2^+/+, Apc2^+/− or Apc2^−/− male crossed with 2 Apc2^+/+, 2 Apc2^+/− or 2 Apc2^−/− female respectively. One Apc2^−/− trio was completely sterile (*P < 0.05). b Breeding efficiency as reflected by number of gestations occurring in a 3 month period (mean ± S.E, n = 4). c Cumulative number of pups weaned in a 3 month period from 4 breeding pairs. Statistical significance between groups was determined using ANOVA test followed by Games-Howell post hoc analysis (variances of experimental groups were not homogeneous, tested by Levene’s test). d, e Representative photomicrographs of (d) Apc2^+/+ and (e) Apc2^−/− ovaries, showing growing follicles (red arrows) and corpora lutea (black arrows). Bar = 500 μm. f, g Histograms showing total number of (f) corpora lutea, and (g) healthy growing follicles, counted across 100 serial sections of four ovaries collected from four animals at diestrus stage (mean ± SE; *P < 0.05, t-test)