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Fig. 5 | BMC Cancer

Fig. 5

From: Sirtuin1 activator SRT2183 suppresses glioma cell growth involving activation of endoplasmic reticulum stress pathway

Fig. 5

Pharmacological inhibition of either NF-κB or STAT3 enhances SRT2183-mediated glioma cell death. a lysates from LN229 and U87MG cells were collected to analyze acetyl-STAT3 and acetyl-NF-κB expression levels by Immunoprecipitation (IP) and IB. The expression levels of total STAT3 and total NF-κB p65 were measured as a loading control. Three independent experiments were used to illustrate the results. b LN229 and U87MG cells were treated with vehicle or 10 μM SRT2183 for 24 h, protein levels of P-NF-κB p65 (Ser536) and P-STAT3 (Y705) were analyzed by IB. LN229 (c) and U87MG (d) cells were treated with vehicle or SRT2183 following pre-treatment with NF-κB inhibitor BAY11–7082 or STAT3 inhibitor C188–9, CCK8 analysis was employed to determine cell growth inhibition. The above experiments were performed three times with comparable results. Results portrayed as the mean ± SEM (***p < 0.001).

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