Skip to main content
Fig. 3 | BMC Cancer

Fig. 3

From: Sirtuin1 activator SRT2183 suppresses glioma cell growth involving activation of endoplasmic reticulum stress pathway

Fig. 3

a ER stress plays a role in SRT2183 promoted glioma cell death. LN229 and U87MG cells were treated with vehicle or 10 μM SRT2183 for 24, 48 h, expression levels of Bip1, PERK, IRE1α, P-EIF2α, and CHOP were analyzed by IB. b LN229 and U87MG cells were pre-treated with vehicle, 4-PBA (100 μM and 500 μM), and then the cells were treated with vehicle or 10 μM SRT2183 for 24, 48 h, after collected the cell lysate, expression levels of Bip1, PERK, and IRE1α were measured by IB. c LN229 and U87MG cells were pre-treated with 4-PBA (500 μM), and then the cells were treated with vehicle or 10 μM SRT2183 for 24, 48, 72 h, cell proliferation was measured by CCK-8 assay. The above experiments were performed three times with comparable results. Results portrayed as the mean ± SEM (**0.001 < p < 0.01, ***p < 0.001).

Back to article page