Fig. 1

SRT2183 suppresses glioma cell growth and lyses neurospheres in vitro. a Modified concentrations of SRT2183 (0.01, 10, 100 μM) were used to treat LN229, SF539, SF767, U87MG, and HUVEC cells with the vehicle for 12, 24 and 48 h. CCK8 analysis displayed the inhibition of cell growth. Results portrayed as the mean ± SEM (***p < 0.001) b Cells (LN229, SF539, SF767, and U87MG) undergo treatment with vehicle control and 10 uM SRT2183 and afterward nourished with complete growth medium 2 weeks to test the number of colonies in a clonogenic assay. Results portrayed as the mean ± SEM (***p < 0.001). c 3D cultures of LN229 and U87MG cells were treated with vehicle or 10 uM SRT2183 for 10 days and examined for the spheroid formation and spheroid diameter (Scale bar = 200um). Results were declared as a number of microspheroids±SEM (***p < 0.001). The above assays were revised three times with identical results.